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Understanding Health Testing and Risk Factors Australian Shepherds

Informed Choices, Healthier Puppies

—Support Responsible Breeders

At High Desert Companions, we believe that responsible breeding begins with knowledge—and that means complete, transparent health testing. Following the gold standards set by the Australian Shepherd Club of America (ASCA), and the Orthopedic Foundation for Animals (OFA), we screen our dogs for all relevant genetic and structural conditions to ensure we’re making the best possible choices for our puppies and their future families.

Understanding inherited diseases, how they work, and what risk factors exist is crucial for ethical breeding. It allows us to make informed pairings that reduce the likelihood of health issues, preserve the integrity of the breed, and support the long-term well-being of every dog we bring into the world.

We’re genuinely pleased that you’re taking the time to research health testing for your future pet and family member—it shows a deep level of care and responsibility. Supporting that kind of initiative is truly my passion. That’s why we’re committed to providing clear, honest information so you feel confident, informed, and fully equipped to make the best decisions for your future companion.

While breeders who fully test their breeding dogs may charge more, the value they offer in peace of mind, quality, and long-term savings on veterinary care is immeasurable. Choosing a breeder who prioritizes health testing not only protects your puppy—it supports the kind of breeding that truly makes a difference.

Health Testing Guidelines
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Do you have an Aussiedoodle mix?
It’s important to remember that all Doodles and Poodle mixes inherit genetic health risks from every breed in their lineage—not just the Aussie or Poodle! To fully understand and manage your dog’s health, you need to research the health conditions and structural traits of all breeds involved in the cross.

Foundations of a Healthy Australian Shepherd:
What to Test and Why

Ethical, responsible breeding begins with science-backed health screening. We follow the recommendations of the Orthopedic Foundation for Animals (OFA) and the Australian Shepherd Club of America (ASCA).

DNA testing allows us to identify carriers of inherited diseases before they are passed to the next generation. Combined with orthopedic screening, this proactive approach protects both our breeding program and the families who trust us with their companions.

Key DNA Tests Recommended for

Australian Shepherds:

  • MDR1 – Multi-Drug Resistance Mutation

Affects the ability to process certain medications. Dogs with this mutation are at risk for severe or fatal reactions to commonly used drugs (like Ivermectin, Loperamide, and some anesthetics).

  • PRA-PRCD – Progressive Retinal Atrophy

A degenerative eye disease that leads to progressive vision loss and eventual blindness.

  • CEA – Collie Eye Anomaly

A congenital eye defect that can cause blindness in severe cases; common in herding breeds including Aussies.

  • HC – Hereditary Cataracts

Causes opacity in the lens, typically developing between 1–3 years of age, and may lead to blindness.

  • DM – Degenerative Myelopathy

A progressive neurological disorder that affects mobility in adult dogs.

  • HUU – Hyperuricosuria

Causes elevated levels of uric acid, which may lead to bladder stones or urinary tract issues.

  • CMR1 – Canine Multifocal Retinopathy

Typically causes retinal abnormalities which can affect vision; some dogs remain symptom-free.

  • NCL – Neuronal Ceroid Lipofuscinosis (Type 6 & 8)

A fatal neurodegenerative disease that causes neurological deterioration in young dogs.

  • ACVO Eye Examination

Performed annually by a board-certified veterinary ophthalmologist, with results registered through OFA.

What the DNA Results Mean:

  • Clear: The dog does not carry the mutation and cannot pass it on.

  • Carrier: The dog carries one copy of the mutation; not typically affected but can produce affected offspring if bred to another carrier.

  • Affected: The dog has two copies of the mutation and is more likely to express the condition.

Orthopedic & Structural Screening:

  • OFA Hip & Elbow Evaluations

Radiographs assessed by board-certified radiologists to detect early signs of dysplasia. OFA certifications are essential for breeding soundness.

  • PennHIP Evaluation (optional, but highly informative)

Measures hip joint laxity to assess early risk for developing hip dysplasia—more precise than standard OFA scoring and highly valuable in an active working breed like the Aussie.

  • OFA Patella Evaluation (Optional but beneficial)

Checks for luxating patella, which although rare in Aussies, is a useful screen especially if smaller lines or mixed heritage is involved.

  • OFA Shoulder Evaluation (Recommended for working or performance lines)

Australian Shepherds are prone to shoulder osteochondritis dissecans (OCD), particularly in active or competitive dogs.

  • OFA Dentition, Cardiac, and Thyroid Evaluations (Optional but informative)

Thyroid issues can impact behavior and fertility. Cardiac and dentition screening can identify abnormalities that affect long-term health or breeding suitability.

Understanding OFA & PennHIP Results:

OFA (Orthopedic Foundation for Animals)

Hips:

  • Excellent: Ideal joint conformation

  • Good: Normal and healthy

  • Fair: Still within normal limits, slightly less ideal

  • Borderline: Re-check advised in 6 months

  • Mild/Moderate/Severe Dysplasia: Not recommended for breeding

Elbows:

  • Normal: No dysplasia present

  • Grade 1, 2, 3 Dysplasia: Increasing severity of arthritic or structural changes

PennHIP (University of Pennsylvania Hip Improvement Program)

Evaluates hip laxity (looseness) using a Distraction Index (DI) from 0 to 1+:

  • Lower DI (closer to 0): Tighter hips, lower risk

  • Higher DI (closer to 1): Looser hips, higher risk

Each DI is compared to the breed average—dogs with a DI lower than the breed median are preferred for breeding.
Example: For Australian Shepherds, a DI below 0.40–0.50 is often considered ideal.

Beyond "Clear on 257 Diseases"

It’s easy to be impressed by claims like “clear on 257 diseases,” but most purebred dogs are only at risk for a small number of those. That’s because genetic mutations are typically limited to a specific breed’s gene pool.

Truly responsible breeding goes beyond DNA.
It means combining genetic screening with orthopedic, structural, and temperament evaluations to prevent issues that could surface later in life.

In the following sections, we’ll take a closer look at each of these conditions—what they are, how they’re inherited, their risk levels, and how you can reduce the chances of your dog being affected through informed care and responsible choices.

DNA tested diseases

MDR1 – Multi-Drug Resistance Mutation

Description:

A mutation in the MDR1 (ABCB1) gene impairs the dog's ability to metabolize certain medications. Affected dogs can have severe or fatal reactions to commonly used drugs, including Ivermectin (heartworm prevention), Loperamide (Imodium), certain sedatives, and chemotherapy agents.

Genotypes & Risk Levels:

  • Clear (N/N): No copies. Safe to receive medications.

  • Carrier (N/MDR1): One copy. Mild sensitivity possible; caution with certain drugs.

  • Affected (MDR1/MDR1): Two copies. High sensitivity. Serious reactions possible.

Lifestyle Management:

  • Always notify your vet about MDR1 status before administering medications.

  • Avoid drugs listed as unsafe by Washington State University’s MDR1 list.

  • Use alternative flea/heartworm preventatives when necessary.

PRA-PRCD – Progressive Retinal Atrophy

More eye information in the exam section below

Description:
An inherited, degenerative eye disorder that causes gradual vision loss due to retinal deterioration. Onset usually occurs between 3–7 years of age.

Genotypes & Risk Levels:

  • Clear (N/N): No mutation. No risk.

  • Carrier (N/PRA): One copy. Unaffected but may pass the gene to offspring.

  • Affected (PRA/PRA): Two copies. High risk for vision loss progression to blindness.

CEA – Collie Eye Anomaly

Description:
A congenital disorder affecting the retina, choroid, and optic nerve. Severity varies—from mild defects to blindness from retinal detachment.

Genotypes & Risk Levels:

  • Clear (N/N): No mutation. No risk.

  • Carrier (N/CEA): One copy. No symptoms but can pass it on.

  • Affected (CEA/CEA): May have mild to severe vision defects.

Lifestyle Management:

  • Conduct ACVO exams early in life (as abnormalities are visible in young puppies).

  • Avoid breeding two carriers or affected dogs.

  • Monitor for worsening vision and provide a stable, safe environment.

HC – Hereditary Cataracts

Description:
Affects the lens of the eye, leading to cloudiness or complete blindness. Typically begins between 1–3 years of age.

Genotypes & Risk Levels:

  • Clear (N/N): No risk.

  • Carrier (N/HC): May never develop cataracts but can pass the gene.

  • Affected (HC/HC): Higher likelihood of cataract development and potential blindness.

Lifestyle Management:

  • Schedule regular eye exams.

  • Monitor for signs like squinting or cloudy eyes.

  • Cataract surgery may be an option in severe cases.

  • Avoid breeding affected dogs.

DM – Degenerative Myelopathy

Description:
A progressive spinal cord disease causing hind limb weakness and eventual paralysis. Onset is usually after 7 years of age.

Genotypes & Risk Levels:

  • Clear (N/N): No risk.

  • Carrier (N/DM): One copy. Not affected, but can pass it on.

  • At Risk (DM/DM): Two copies. Increased chance of developing symptoms, though not all will.

Lifestyle Management:

  • Maintain a lean body condition.

  • Engage in low-impact exercise.

  • Consider physical therapy as symptoms emerge.

  • Prompt evaluation of early signs like stumbling or dragging feet.

HUU – Hyperuricosuria

Description:
A metabolic disorder leading to high uric acid levels in urine. Increases risk of bladder stones and urinary tract infections.

Genotypes & Risk Levels:

  • Clear (N/N): No mutation.

  • Carrier (N/HUU): One copy. No symptoms but can pass on the gene.

  • Affected (HUU/HUU): Risk of bladder/kidney stones.

Lifestyle Management:

  • Feed a low-purine diet.

  • Ensure constant access to fresh water.

  • Regularly monitor urine pH and urinalysis.

  • Avoid breeding two carriers or affected dogs.

CMR1 – Canine Multifocal Retinopathy

Description:
A retinal disorder causing raised lesions on the eye. May affect vision, but many dogs remain symptom-free.

Genotypes & Risk Levels:

  • Clear (N/N): No mutation.

  • Carrier (N/CMR1): One copy. No symptoms, may pass it on.

  • Affected (CMR1/CMR1): Retinal changes; may have some vision impairment.

Lifestyle Management:

  • Annual eye exams can track changes.

  • Most dogs do not need treatment.

  • Use caution in breeding—avoid affected pairings.

NCL (Neuronal Ceroid Lipofuscinosis) Types 6 & 8

Description:
A fatal, inherited neurodegenerative disease. Causes progressive neurological symptoms starting in early life (as early as 6 months), including motor decline, seizures, and eventual death.

Genotypes & Risk Levels:

  • Clear (N/N): Safe.

  • Carrier (N/NCL): No symptoms, but a carrier.

  • Affected (NCL/NCL): Fatal disease. Symptoms begin early and worsen quickly.

Lifestyle Management:

  • No treatment—prevention is key.

  • Puppies with NCL are typically euthanized by 1–2 years due to suffering.

  • Never breed two carriers.

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PRA & ACVO Eye Exam
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PRA-PRCD – Progressive Retinal Atrophy (Progressive Rod-Cone Degeneration)

A Genetic Guide for Vision Health

Progressive Retinal Atrophy – Progressive Rod-Cone Degeneration (PRA-PRCD) is a hereditary, degenerative disease that affects the retina—the light-sensitive layer at the back of the eye. In affected dogs, the photoreceptor cells (rods and cones) deteriorate over time, leading to night blindness, and eventually, complete vision loss. Onset in Australian Shepherds often begins between 3 and 7 years of age, though it can vary.

PRA-PRCD is inherited in an autosomal recessive pattern, meaning both parents must be carriers to produce affected puppies.

 

Genotypes & Risk Levels in Australian Shepherds

  • Clear (N/N): ~70–80% of Australian Shepherds
    The dog does not carry the PRA-PRCD mutation.
    No risk of developing or passing on the disease.

  • Carrier (N/PRA): ~15–25% Carries one copy of the mutated gene.
    Will not develop PRA but can pass the gene to offspring.
    Safe to breed only with Clear (N/N) mates to avoid producing affected puppies.

  • Affected (PRA/PRA): <1–2% Have two copies of the mutated gene.
    Dogs that have two copies of the mutated gene will develop PRA.
    Vision loss is progressive, but not painful. Dogs typically adapt well with support.

 

Lifestyle Support for PRA-Affected Aussies

For PRA/PRA dogs or those showing signs of vision loss:

Environmental Adjustments:

  • Keep furniture layout consistent.

  • Use textured mats or rugs to mark pathways and key areas.

  • Install baby gates to prevent falls on stairs.

Safety & Enrichment:

  • Keep the dog leashed in unfamiliar areas.

  • Introduce scent-marked toys and food puzzles to stimulate other senses.

  • Use voice or gentle touch cues to help guide them.

Veterinary Monitoring:

  • Schedule regular eye exams to track progression.

  • Nutritional support like omega-3s, lutein, and antioxidants may help overall eye health.

 

Breeding Considerations

  • Do not breed affected dogs (PRA/PRA).

  • Carriers (N/PRA) may be bred to Clear (N/N) mates only.

  • Always test both members of a breeding pair.

  • Maintain diversity while eliminating the risk of producing blind puppies.

 

ACVO Eye Examination in Australian Shepherds

Protecting Vision, Preserving Bloodlines

Australian Shepherds are predisposed to several heritable eye disorders. Annual eye exams by a board-certified veterinary ophthalmologist play a key role in maintaining long-term breed health and vision.

 

What Is the ACVO Eye Exam?

This exam, conducted by a board-certified veterinary ophthalmologist, screens for inherited ocular diseases such as:

  • PRA (Progressive Retinal Atrophy)

  • Collie Eye Anomaly (CEA)

  • Cataracts

  • Distichiasis (extra eyelashes)

  • Persistent Pupillary Membranes

  • Retinal folds/dysplasia

  • Iris colobomas

  • Microphthalmia

  • Glaucoma risk factors

The exam involves pupil dilation and a detailed inspection of all eye structures using specialized equipment.

 

Why It Matters for Australian Shepherds

Australian Shepherds—especially those from working and performance lines—are known carriers of multiple hereditary eye disorders, including CEA and PRA.

Even dogs that appear visually normal may silently carry genes that can pass on serious conditions.

Routine ACVO exams help breeders:

  • Confirm dogs are free of inherited ocular conditions.

  • Make informed, ethical breeding choices.

  • Promote healthier litters and long-term breed sustainability.

 

Registering with OFA (Orthopedic Foundation for Animals)

  • Once the ACVO exam is complete, the results can be submitted to OFA’s public health database.

  • Owners receive a certification.

  • Dogs receive a CERF number, though CERF is now integrated into OFA.

  • 🗓 Annual exams are recommended, as many eye diseases are progressive.

 

ACVO + Genetic Testing = Full Picture

The ACVO exam identifies visible, developing, or structural eye diseases—but it does not detect genetic carriers.
To get a full view of vision health:

Pair annual ACVO exams with DNA testing for PRA-PRCD and CEA (Collie Eye Anomaly).
Use both tests to identify clear, carrier, and affected dogs.
Breed only combinations that will not produce affected puppies.

By protecting their vision today, you’re preserving the breed’s legacy for years to come.

Ortopedic Screening For Poodles

Orthopedic Screening for
Australian ShepherdWhy It Matters

Orthopedic health is a critical aspect of responsible breeding and long-term care in Australian Shepherds. While these energetic and intelligent dogs are beloved for their athleticism and drive, they can be genetically predisposed to joint and skeletal issues, particularly in the hips, elbows, and shoulders. Early detection through orthopedic screening supports ethical breeding, ensures sound movement, and promotes lifelong mobility.

Essential Orthopedic Evaluations for Australian Shepherds

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OFA Hip & Elbow Evaluations

What it is:
Standard radiographic screening evaluated by three board-certified radiologists through the Orthopedic Foundation for Animals (OFA). Results are graded for conformation and any signs of hip or elbow dysplasia.

When it’s done:

  • Official OFA Certification: 24 months and older

  • Preliminary Evaluation: Available from 4 months of age for early insight

Why it matters:
Hip and elbow dysplasia can be painful and debilitating. Detecting signs early allows breeders to make informed decisions and owners to plan supportive care strategies.

PennHIP Evaluation

What it is:
A precise radiographic method developed by the University of Pennsylvania to measure hip joint laxity. It quantifies risk for hip dysplasia through a Distraction Index (DI).

Why it’s more precise:

  • Detects risk as early as 16 weeks old

  • Offers a numerical DI score rather than subjective grading

  • Identifies subtle joint looseness before dysplasia develops

Ideal for:

  • All breeding dogs

  • High-drive, working, or performance Aussies

OFA Shoulder Evaluation (Optional)

What it checks:
Orthopedic screening for Osteochondritis Dissecans (OCD)—a developmental joint disease that affects the shoulder cartilage, sometimes seen in medium-to-large breeds including Aussies.

Who should be tested:

  • Performance dogs

  • Dogs with known OCD in pedigree

  • Breeding stock from lines with previous shoulder issues

OFA Eye Certification (ACVO Exam)

What it is:
Annual eye examination performed by a veterinary ophthalmologist, looking for structural or hereditary eye conditions (e.g., collie eye anomaly, cataracts).

Why it matters:
Australian Shepherds are genetically predisposed to several eye issues that may impact vision or quality of life.

OFA (Orthopedic Foundation for Animals) Grading Summary

Hips:

  • Excellent: Tight, well-formed joints

  • Good: Normal, healthy joint formation

  • Fair: Slightly less ideal but still within acceptable range

  • Borderline: Recheck recommended in 6 months

  • Mild/Moderate/Severe Dysplasia: Joint abnormalities—not recommended for breeding

Elbows:

  • Normal: No dysplasia detected

  • Grade 1–3: Increasing severity of joint degeneration or abnormalities

PennHIP (University of Pennsylvania Hip Improvement Program)

Distraction Index (DI):

  • Lower DI (closer to 0): Tighter hips, lower lifetime risk

  • Higher DI (closer to 1): Looser hips, higher lifetime risk

Benchmark for Australian Shepherds:
DI under 0.30–0.40 is typically ideal. Dogs with DI below the breed median are better suited for breeding.

How to Look Up OFA Scores &

Evaluate Orthopedic Health in Breeding Dogs

 

Orthopedic screening—particularly for hip and elbow dysplasia, patellas, and Legg-Calvé-Perthes Disease—is an essential part of selecting quality breeding dogs. But evaluating a dog’s health isn’t just about checking their individual scores. You should also assess the consistency of sound orthopedic health in their lineage, including siblings, parents, grandparents, and even aunts/uncles.

Here’s how to do it step by step:

Step 1: Visit the OFA Website

  • Go to www.ofa.org

  • Click on the “Search OFA Records” tab at the top.

  • You can search by:

    • Dog’s registered name

    • Dog’s registration number

    • Partial name or kennel prefix (if you’re browsing lines)

Tip: Use the dog’s full AKC-registered name for the most accurate results.

Step 2: Review the Dog’s OFA Record Page

Once you’ve selected a dog, you’ll see a page with a list of all recorded health tests. Focus on the orthopedic categories:

Key Evaluations:

  • Hips – Rated as:

    • Excellent

    • Good

    • Fair

    • Borderline

    • Mild/Moderate/Severe Dysplasia (these indicate a failed certification)

  • Elbows – Normal or Grade 1–3 (1–3 indicating dysplasia)

  • Patellas – Normal or Grades 1–4 (grades indicate increasing severity of luxation)

  • Shoulders (Normal or affected, if tested)

Each record will show:

  • Age at time of evaluation

  • Final result

  • Registry (OFA, PennHIP, etc.)

  • If radiographs were preliminary or official

Step 3: Examine the Dog’s Relatives

At the bottom of the OFA record, you’ll find a "Vertical Pedigree"—this is an essential tool for evaluating risk from a genetic standpoint.

What to look for:

  • Consistency of good scores (e.g., Good or Excellent hips) in:

    • Both parents

    • Siblings (especially full littermates)

    • Grandparents

    • Aunts, uncles, half-siblings

  • Avoid lines where:

    • Most littermates or parents have “Fair” or below scores.

    • There’s a pattern of failed hip or elbow certifications in close relatives.

    • One “Excellent” is surrounded by a sea of “Poor” relatives—that’s often a statistical outlier.

Why This Matters:

Genetics is about probability. Even if the individual dog has an “Excellent” rating, if its parents or siblings have mild or severe dysplasia, that dog still carries genetic material likely to pass on orthopedic problems.

Example:
If two parents produce one pup with Excellent hips and three with Moderate dysplasia, it means the genetic background is unstable. That excellent pup may look like a good candidate but is statistically more likely to pass on bad hips compared to a dog whose litter and family consistently have Good/Excellent scores.

Step 4: Cross-reference with PennHIP (if available)

If the breeder also shares PennHIP results, review the Distraction Index (DI):

  • A lower DI (0.3–0.4 or less) means tighter hips and lower risk.

  • A higher DI (>0.5) suggests looser hips and increased risk, even if the dog hasn’t yet developed dysplasia.

  • Look for consistency across generations here, too.

Final Tips for Breeding Soundness

  • Don’t be fooled by a single score. Look at the pattern of health across 2–3 generations.

  • Breeders aiming to improve the breed prioritize lines with:

    • Low, consistent DI scores

    • Multiple generations of Excellent or Good hips

    • Normal elbows, patellas, and skeletal health

  • Always combine orthopedic data with overall conformation, temperament, and breed-specific health testing.

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